A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities

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A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities
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Keywords

Antibacterial activity
Antibiofilm activity
Anticancer activity
Biofilm inhibition
Biomedical applications
Clove oil
Essential oils
DLS (Dynamic Light Scattering)
Nanoemulsion
TEM (Transmission Electron Microscopy)
Thyme oil

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How to Cite

1.
Hashem AH, Doghish ASD, Ismail A, Hassanin MM, Okla MK, Saleh IA, AbdElgawad H, Shehabeldine AM. A novel nanoemulsion based on clove and thyme essential oils: Characterization, antibacterial, antibiofilm and anticancer activities. Electron. J. Biotechnol. [Internet]. 2024 Mar. 15 [cited 2024 Oct. 13];68:20-3. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2357

Abstract

Background: Essential oil nanoemulsions have received much attention in the last period due to their ability to fight microbes and cancers. In the current study, clove and thyme essential oils CL+TH-emulsion and CL+TH-nanoemulsion were prepared through an eco-friendly method. The prepared CL+TH-nanoemulsion was characterized using DLS and TEM analyses.

Results: Results revealed that CL+TH-nanoemulsion droplets were spherical in shape and nanoform in size (68.6 nm) with PDI 0.281. MIC concentrations of CL+TH-nanoemulsion against tested bacteria were found to be between 6.25 and 25 mg/mL. After being exposed to MICs of CL+TH-emulsion and CL+TH-nanoemulsion, which additionally prompted 1.43 log and 3.12 log declines, accordingly, as opposed to untreated (Control), the number of cells grown in the generated biofilms decreased. Furthermore, CL+TH-nanoemulsion exhibited anticancer activity more than CL+TH-emulsion toward HepG2 and MCF-7. Also, the effect of CL+TH-nanoemulsion is more effective and significantly cytotoxic than taxol on MCF-7. Besides, both prepared emulsions increased the rate of apoptosis and decreased the cell viability % of MCF-7 by increasing the activity of caspases 8 and 9. Moreover, CL+TH-nano emulsion decreased the activity of VEGFR-2 in MCF-7 in a more pronounced manner than CL+TH-emulsion and taxol.

Conclusions: The prepared CL+TH-nanoemulsion had antibacterial, and antibiofilm as well as anticancer properties, which can be used in different biomedical applications after extensive studies in vivo.

https://doi.org/10.1016/j.ejbt.2023.12.001
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References

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