Callus induction and plant regeneration of Ulex europaeus
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Keywords

plant regeneration
callus
Ulex europaeus
gorse
Fabaceae

How to Cite

1.
Ramírez I, Dorta F, Cuadros-Inostroza A, Peña-Cortés H. Callus induction and plant regeneration of Ulex europaeus. Electron. J. Biotechnol. [Internet]. 2012 Jun. 26 [cited 2024 Nov. 6];15(4). Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v15n4-4

Abstract

A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.    

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