Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum
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Keywords

jasmonic acid
wounding
12-oxophytodienoate reductase
Solanum tuberosum
stress
OPDA

How to Cite

1.
Díaz M, Polanco V, Ramírez I, Peña-Cortés H. Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum. Electron. J. Biotechnol. [Internet]. 2011 Nov. 23 [cited 2024 Oct. 13];15(1). Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v15n1-3

Abstract

Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene.

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