Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
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Keywords

chilling response
peanut
real-time quantitative PCR
suppression subtractive hybridization

How to Cite

1.
Tang YY, Wang CT, Yang GP, Feng T, Gao HY, Wang XZ, Chi XY, Xu YL, Wu Q, Chen DX. Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.). Electron. J. Biotechnol. [Internet]. 2011 Sep. 15 [cited 2024 Nov. 6];14(5). Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v14n5-5

Abstract

To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).

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