Isolation of simple sequence repeats from groundnut
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Keywords

groundnut
isolation
simple sequence repeat

How to Cite

1.
Wang CT, Yang XD, Chen DX, Yu SL, Liu GZ, Tang YY, Xu JZ. Isolation of simple sequence repeats from groundnut. Electron. J. Biotechnol. [Internet]. 2007 Jul. 15 [cited 2024 Oct. 13];10(3):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v10n3-10

Abstract

SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts.

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