Efficient immobilization of agarase using carboxyl-functionalized magnetic nanoparticles as support
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Keywords

agar-derived oligosaccharides
agaro-oligosaccharide
dynamic light scattering
enzyme characterization
scanning electron microscopy
thermogravimetric analysis
transmission electron microscopy
vibrating sample magnetometry
X-ray diffraction
zeta-pot

How to Cite

1.
Xiao A, Xiao Q, Lin Y, Ni H, Zhu Y, Cai H. Efficient immobilization of agarase using carboxyl-functionalized magnetic nanoparticles as support. Electron. J. Biotechnol. [Internet]. 2017 Jan. 12 [cited 2024 Oct. 8];25. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2016.10.007

Abstract

Background: A simple and efficient strategy for agarase immobilization was developed with carboxyl-functionalized magnetic nanoparticles (CMNPs) as support. The CMNPs and immobilized agarase (agarase-CMNPs) were characterized by transmission electron microscopy, dynamic light scattering, vibrating sample magnetometry, scanning electron microscopy, X-ray diffraction, thermogravimetric analysis, and zeta-potential analysis. The hydrolyzed products were separated and detected by ESI-TOF-MS.

Results: The agarase-CMNPs exhibited a regular spherical shape with a mean diameter of 12 nm, whereas their average size in the aqueous solution was 43.7 nm as measured by dynamic light scattering. These results indicated that agarase-CMNPs had water swelling properties. Saturation magnetizations were 44 and 29 emu/g for the carriers and agarase-CMNPs, respectively. Thus, the particles had superparamagnetic characteristics, and agarase was successfully immobilized onto the supports. Agaro-oligosaccharides were prepared with agar as substrate using agarase-CMNPs as biocatalyst. The catalytic activity of agarase-CMNPs was unchanged after six reuses. The ESI-TOF mass spectrogram showed that the major products hydrolyzed by agarase-CMNPs after six recycle uses were neoagarotetraose, neoagarohexaose, and neoagarooctaose. Meanwhile, the end-products after 90 min of enzymatic treatment by agarase-CMNPs were neoagarobiose and neoagarotetraose.

Conclusions: The enhanced agarase properties upon immobilization suggested that CMNPs can be effective carriers for agarase immobilization. Agarase-CMNPs can be remarkably used in developing systems for repeated batch production of agar-derived oligosaccharides.
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