Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
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Keywords

catalytic antibodies
in vitro cultures
molecular farming
recombinant antibodies
tobacco

How to Cite

1.
Martínez C, Petruccelli S, Giulietti AM, Alvarez MA. Expression of the antibody 14D9 in Nicotiana tabacum hairy roots. Electron. J. Biotechnol. [Internet]. 2005 Aug. 15 [cited 2024 Dec. 11];8(2):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n2-10

Abstract

Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively.

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