Recombinant expression of marine shrimp lysozyme in Escherichia coli
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Keywords

crustacea
lysozyme
penaeideae
Penaeus vannamei
recombinant expression
refolding
shrimp

How to Cite

1.
de-la-Re-Vega E, García-Orozco KD, Calderón-Arredondo SA, Romo-Figueroa MG, Islas-Osuna MA, Yepiz-Plascencia GM, Sotelo-Mundo RR. Recombinant expression of marine shrimp lysozyme in Escherichia coli. Electron. J. Biotechnol. [Internet]. 2004 Dec. 15 [cited 2024 Oct. 13];7(3):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v7n3-3

Abstract

Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp (Penaeus vannamei) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10%. Details of the protein recombinant expression techniques applied to this shrimp protein are presented.

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