Agrobacterium tumefaciens: a natural tool for plant transformation
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Keywords

Agrobacterium tumefaciens
T-DNA
Plant transformation

How to Cite

1.
de la Riva GA, González-Cabrera J, Vázquez-Padrón R, Ayra-Pardo C. Agrobacterium tumefaciens: a natural tool for plant transformation. Electron. J. Biotechnol. [Internet]. 1998 Dec. 15 [cited 2024 Oct. 13];1(3):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v1n3-1

Abstract

Updated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.

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