Optimization of β-carotene production by a newly isolated Serratia marcescens strain
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Keywords

optimization
pigment
Serratia marcescens.

How to Cite

1.
Wang B, Lin L, Lu L, Chen W. Optimization of β-carotene production by a newly isolated Serratia marcescens strain. Electron. J. Biotechnol. [Internet]. 2012 Oct. 19 [cited 2024 Dec. 3];15(6). Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v15n6-3

Abstract

β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 µg/mL.
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