Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
Full Text
Reprint PDF

Keywords

diffusional restrictions
enzyme kinetics
immobilized enzyme
penicillin G acylase
penicillin hydrolysis

How to Cite

1.
Illanes A, González JM, Gómez JM, Valencia P, Wilson L. Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading. Electron. J. Biotechnol. [Internet]. 2010 Jan. 15 [cited 2024 Dec. 4];13(1):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v13n1-12

Abstract

Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10-5m and 10·10-4m at protein loadings from 15 to 130 mg/ggel. Internal diffusional restrictions were evaluated for such biocatalysts: Thiele modulus varied from 1.17 for the small particles at the lower protein load to 5.84 for the large particles at the higher protein load. Effectiveness factors at different bulk substrate concentrations were determined for all biocatalysts, values ranging from 0.78 for small particle size at 25 mM penicillin G to 0.15 for large particle size at 2 mM penicillin G. Enzyme protein loading had a strong impact on the effectiveness factors of immobilized penicillin G acylase, being it more pronounced in the case of large particle size biocatalysts. At conditions in which 6-aminopenicillanic acid is industrially produced, all biocatalysts tested were mass-transfer limited, being this information valuable for reactor design and performance evaluation.

Full Text
Reprint PDF

Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".

The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.