Measuring β-Galactosidase activity at pH 6 with a differential pH sensor
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Keywords

delta milli pH
differential pH

How to Cite

1.
Acevedo CA, Stach MH, Amtmann A, Young ME, Reyes JG, Huebner H, Buchholz R. Measuring β-Galactosidase activity at pH 6 with a differential pH sensor. Electron. J. Biotechnol. [Internet]. 2009 Apr. 15 [cited 2024 Nov. 12];12(2):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v12n2-10

Abstract

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

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