Abstract
Background: Coffee fermentation process influences the final coffee composition and the sensory aspects which define the quality of the beverage. In this study, coffee fruits underwent spontaneous self-induced anaerobic fermentation using samples with two percentages of immature fruits in submerged and solid-state processing. The effects on the physicochemical composition and sensory quality of coffees were evaluated
Results: The two percentages of immature fruits corresponded to 11.0 and 0.3% of unripe fruits. The percentage of immature fruits significantly altered the initial content of sugars (sucrose, glucose, and fructose), ash, and titratable acidity. The water addition during the fermentative process did not significantly influence final moisture, proteins, citric acid, and propionic acid concentrations. Compared to the solid-state, the submerged process gave rise to coffees with lower concentrations of ethanol, glycerol, ash, lipids, succinic, and acetic acids. Coffee fermented with 0.3% of immature fruits showed higher lactic acid production in submerged fermentation (67.44 mg/g), and higher concentrations of ethanol (42.84 mg/g) and glycerol (1.68 mg/g) in solid-state fermentation. All coffees produced were classified as specialty coffees with a score above 84 points. However, the submerged fermented coffee with 11% immature fruit stood out with notes of caramel, brown sugar, honey, orange, lemon, floral, nut, yellow and red fruits.
Conclusions: This study confirmed that spontaneous fermentation can be used to produce specialty coffees. Differentiation in sensory attributes can be achieved through the addition of water and varying the percentage of green fruits during the fermentation process. Up to 11% of immature fruits did not compromise coffee quality.
References
Pereira GVM, Carvalho Neto DP, Magalhães Júnior AI, et al. Exploring the impacts of postharvest processing on the aroma formation of coffee beans – A review. Food Chem 2019;272:441–452. https://doi.org/10.1016/j.foodchem.2018.08.061 PMid: 30309567
Santos ÉM, Macedo LM, Tundisi LL, et al. Coffee by-products in topical formulations: A review. Trends Food Sci Technol 2021;111:280–291. https://doi.org/10.1016/j.tifs.2021.02.064
Elías L. Composición química de la pulpa de café y otros subproductos [Chemical composition of coffee pulp and other by-products]. In: Braham JE editor. Pulpa de café: Composición, tecnología y utilización [Coffee pulp: Composition, technology and use]. Instituto de Nutrición de Centro América y Panamá, Ciudad de Guatemala, Guatemala; 1978, p. 19-29. (In Spanish).
Schwan RF, Wheals AE. Mixed microbial fermentations of chocolate and coffee. In: Boekhout T, Robert V, editors. Yeasts in Food. Cambridge: Woodhead Publishing; 2003, p. 429–449. https://doi.org/10.1533/9781845698485.429
Pereira LL, Brioschi Júnior D, Sousa LHBP, et al. Relationship between coffee processing and fermentation. In: Pereira LL, Moreira TR, editors. Quality determinants in coffee production. Cham: Springer; 2021, p. 255-301. https://doi.org/10.1007/978-3-030-54437-9_6
Mussatto SI, Machado EMS, Martins S, et al. Production, composition, and application of coffee and its industrial residues. Food and Bioprocess Technology 2011;4:661–672. https://doi.org/10.1007/s11947-011-0565-z
Lee LW, Cheong MW, Curran P, et al. Coffee fermentation and flavor – An intricate and delicate relationship. Food Chem 2015;185:182–191. https://doi.org/10.1016/j.foodchem.2015.03.124 PMid: 25952856
Malta MR, Santos ML, Silva, FAM. Qualidade de grãos de diferentes cultivares de cafeeiro (Coffea arábica L.) [Beans quality of various coffee plant (Coffea arabica L.) cultivars]. Acta Scientiarum Agronomy, 2008;24:1385-1390. (In Portuguese). https://doi.org/10.4025/actasciagron.v24i0.2386
Buitrago-Osorio J, Tinoco HA, Perdomo-Hurtado L, et al. Physical-mechanical characterization of coffee fruits Coffea arabica L. var. Castillo classified by a colorimetry approach. Materialia 2022;21:101330. https://doi.org/10.1016/J.MTLA.2022.101330
Silva ACR, Silva CC, Garrett R, et al. Comprehensive lipid analysis of green Arabica coffee beans by LC-HRMS/MS. Food Research International 2020;137:109727. https://doi.org/10.1016/j.foodres.2020.109727 PMid: 33233296
Bastian F, Hutabarat OS, Dirpan A, et al. From plantation to cup: Changes in bioactive compounds during coffee processing. Foods 2021;10(11):2827. https://doi.org/10.3390/foods10112827 PMid: 34829108
Vilela DM, Pereira GVM, Silva CF, et al. Molecular ecology and polyphasic characterization of the microbiota associated with semi-dry processed coffee (Coffea arabica L.). Food Microbiol 2010;27(8):1128–1135. https://doi.org/10.1016/j.fm.2010.07.024 PMid: 20832694
Silva JS, Moreli AP, Donzeles SML, et al. Harvesting, drying and storage of coffee. In: Pereira LL, Moreira TR, editors. Quality determinants in coffee production. Cham: Springer; 2021, p. 1-64. https://doi.org/10.1007/978-3-030-54437-9_1
Mazzafera, P. Chemical composition of defective coffee beans. Food Chem 1999;64(4):547–554. https://doi.org/10.1016/S0308-8146(98)00167-8
Velásquez S, Peña N, Bohórquez JC, et al. Volatile and sensory characterization of roast coffees – Effects of cherry maturity. Food Chem 2019;274:137–145. https://doi.org/10.1016/j.foodchem.2018.08.127 PMid: 30372918
Elhalis H, Cox J, Frank D, et al. The role of wet fermentation in enhancing coffee flavor, aroma and sensory quality. European Food Research and Technology 2020;247:485–498. https://doi.org/10.1007/s00217-020-03641-6
Pereira TS, Batista NN, Pimenta LPS, et al. Self-induced anaerobiosis coffee fermentation: Impact on microbial communities, chemical composition and sensory quality of coffee. Food Microbiol 2022;103:103962. https://doi.org/10.1016/j.fm.2021.103962 PMid: 35082079
Ribeiro LS, Evangelista SR, Miguel MGCP, et al. Microbiological and chemical-sensory characteristics of three coffee varieties processed by wet fermentation. Annals of Microbiology 2018;68:705–716. https://doi.org/10.1007/s13213-018-1377-4
Cardoso WS, Agnoletti BZ, Freitas R, et al. Biochemical aspects of coffee fermentation. In: Pereira LL, Moreira TR, editors. Quality determinants in coffee production. Cham: Springer; 2021, p. 149–208. https://doi.org/10.1007/978-3-030-54437-9_4
Cortés-Macías ET, Fuentes López C, Gentile P, et al. Impact of post-harvest treatments on physicochemical and sensory characteristics of coffee beans in Huila, Colombia. Postharvest Biol Technol 2022;187:111852. https://doi.org/10.1016/j.postharvbio.2022.111852
Knopp S, Bytof G, Selmar D. Influence of processing on the content of sugars in green Arabica coffee beans. European Food Research and Technology 2006;223:195–201. https://doi.org/10.1007/S00217-005-0172-1
Peñuela-Martínez AE, Zapata-Zapata AD, Durango-Restrepo DL. Performance of different fermentation methods and the effect on coffee quality (Coffea arabica L.). Coffee Sci 2018;13(4):465–476. https://doi.org/10.25186/cs.v13i4.1486
Silva MCS, Luz JMR, Veloso TGR, et al. Processing techniques and microbial fermentation on microbial profile and chemical and sensory quality of the coffee beverage. European Food Research and Technology 2022;248:1499–1512. https://doi.org/10.1007/s00217-022-03980-6
Bressani APP, Martinez SJ, Batista NN, et al. Co-inoculation of yeasts starters: A strategy to improve quality of low altitude Arabica coffee. Food Chem 2021;361:130133. https://doi.org/10.1016/j.foodchem.2021.130133 PMid: 34082390
Cassimiro DMJ, Batista NN, Fonseca HC, et al. Coinoculation of lactic acid bacteria and yeasts increases the quality of wet fermented Arabica coffee. Int J Food Microbiol 2022;369:109627. https://doi.org/10.1016/j.ijfoodmicro.2022.109627 PMid: 35305516
Martins PMM, Batista NN, Miguel MGCP, et al. Coffee growing altitude influences the microbiota, chemical compounds and the quality of fermented coffees. Food Research International 2020;129:108872. https://doi.org/10.1016/j.foodres.2019.108872 PMid: 32036899
Mota MCB, Batista NN, Rabelo MHS, et al. Influence of fermentation conditions on the sensorial quality of coffee inoculated with yeast. Food Research International 2020;136:109482. https://doi.org/10.1016/j.foodres.2020.109482 PMid: 32846564
Association of Official Analytical Chemists (AOAC). Helrich K, editor. Official methods of analysis. 15th ed. Arlington: Association of Official Analytical Chemists Inc; 1990.
Elhalis H, Cox J, Zhao J. Ecological diversity, evolution and metabolism of microbial communities in the wet fermentation of Australian coffee beans. Int J Food Microbiol 2020;321:108544. https://doi.org/10.1016/j.ijfoodmicro.2020.108544 PMid: 32086129
Specialty Coffee Association (SCA). Coffee Standards. California: Specialty Coffee Association; 2018.
Mesquita CM, Rezende JE, Carvalho JS, et al. Manual do Café: Colheita e Preparo [Coffee manual: Harvesting and Preparation (Coffea arabica L.)] Belo Horizonte: Emater; 2016. (In Portuguese).
Pinheiro PF, Pinheiro CA, Osório VM, et al. Chemical constituents of coffee. In: Pereira LL, Moreira TR, editors. Quality determinants in coffee production. Cham: Springer; 2021, p. 209–254. https://doi.org/10.1007/978-3-030-54437-9_5
Rincon-Jimenez A, Tinoco HA, Buitrago-Osorio J, et al. Ripeness stage characterization of coffee fruits (Coffea arabica L. var. Castillo) applying chromaticity maps obtained from digital images. Mater Today Proc 2021;44(Part 1):1271–1278. https://doi.org/10.1016/J.MATPR.2020.11.264
Ribeiro LS, Miguel MGCP, Evangelista SR, et al. Behavior of yeast inoculated during semi-dry coffee fermentation and the effect on chemical and sensorial properties of the final beverage. Food Research International 2017;92:26–32. https://doi.org/10.1016/j.foodres.2016.12.011 PMid: 28290294
Evangelista SR, Miguel MGCP, Silva CF, et al. Microbiological diversity associated with the spontaneous wet method of coffee fermentation. Int J Food Microbiol 2015;210:102–112. https://doi.org/10.1016/j.ijfoodmicro.2015.06.008 PMid: 26119187
Geromel C, Ferreira LP, Guerreiro SMC, et al. Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. J Exp Bot 2006;57(12):3243–3258. https://doi.org/10.1093/jxb/erl084 PMid: 16926239
Janissen B, Huynh T. Chemical composition and value-adding applications of coffee industry by-products: A review. Resour Conserv Recycl 2018;128:110–117. https://doi.org/10.1016/j.resconrec.2017.10.001
Torres DF, Isquierdo EP, Borém FM, et al. Total titratable acidity contents in immature arabica coffee fruits. In: 36° Brazilian Coffee Research Congress. Guarapari: SBICafé; 2010, p.1-2. (In Portuguese).
Prates Júnior P, Veloso TGR, Silva MCS, et al. Soil microorganisms and quality of the coffee beverage. In: Pereira LL, Moreira TR, editors. Quality determinants in coffee production. Cham: Springer; 2021, p. 101–147. https://doi.org/10.1007/978-3-030-54437-9_3
Yeager SE, Batali ME, Guinard JX, et al. Acids in coffee: A review of sensory measurements and meta-analysis of chemical composition. Food Science and Nutrition 2021;63(8):1010-1036. https://doi.org/10.1080/10408398.2021.1957767 PMid: 34553656
Smrke S, Kroslakova I, Gloess AN, et al. Differentiation of degrees of ripeness of Catuai and Tipica green coffee by chromatographical and statistical techniques. Food Chem 2015;174:637–642. https://doi.org/10.1016/j.foodchem.2014.11.060 PMid: 25529730
Mathieu F, Malosse C, Frérot B. Identification of the volatile components released by fresh coffee berries at different stages of ripeness. J Agric Food Chem 1998;46(3):1106–1110. https://doi.org/10.1021/jf970851z
Ni D, Chen Z, Tian Y, et al. Comprehensive utilization of sucrose resources via chemical and biotechnological processes: A review. Biotechnol Adv 2022;60:107990. https://doi.org/10.1016/j.biotechadv.2022.107990 PMid: 3564081
Evangelista SR, Silva CF, Miguel MGPC, et al. Improvement of coffee beverage quality by using selected yeasts strains during the fermentation in dry process. Food Research International 2014;61:183–195. https://doi.org/10.1016/j.foodres.2013.11.033
Hall RD, Trevisan F, Vos RCH. Coffee berry and green bean chemistry – Opportunities for improving cup quality and crop circularity. Food Research International 2022;151:110825. https://doi.org/10.1016/j.foodres.2021.110825 PMid: 34980376
Bressani APP, Martinez SJ, Sarmento ABI, et al. Organic acids produced during fermentation and sensory perception in specialty coffee using yeast starter culture. Food Research International 2020;128:108773. https://doi.org/10.1016/j.foodres.2019.108773 PMid: 31955746
Silva CF, Vilela DM, Cordeiro CS, et al. Evaluation of a potential starter culture for enhance quality of coffee fermentation. World J Microbiol Biotechnol 2013;29:235–247. https://doi.org/10.1007/s11274-012-1175-2 PMid: 23054699
Xu Z, Shi Z, Jiang L. Acetic and propionic acids. In: Moo-Young M, editor. Comprehensive Biotechnology. 2nd ed. Oxford: Pergamon; 2011, p. 189–199. https://doi.org/10.1016/B978-0-08-088504-9.00162-8
Eaton DC, Gabelman A. Fed-batch and continuous fermentation of Selenomonas ruminantium for natural propionic, acetic and succinic acids. J Ind Microbiol 1995;15(1):32–38. https://doi.org/10.1007/BF01570010
Song H, Lee SY. Production of succinic acid by bacterial fermentation. Enzyme Microb Technol 2006;39(3):352–361. https://doi.org/10.1016/j.enzmictec.2005.11.043
Ahn JH, Jang YS, Lee SY. Production of succinic acid by metabolically engineered microorganisms. Curr Opin Biotechnol 2016;42:54–66. https://doi.org/10.1016/j.copbio.2016.02.034 PMid: 26990278
Vicente J, Baran Y, Navascués E, et al. Biological management of acidity in wine industry: A review. Int J Food Microbiol 2022;375:109726. https://doi.org/10.1016/j.ijfoodmicro.2022.109726 PMid: 35635990
Pereira GVM, Neto E, Soccol VT, et al. Conducting starter culture-controlled fermentations of coffee beans during on-farm wet processing: Growth, metabolic analyses and sensorial effects. Food Research International 2015;75:348–356. https://doi.org/10.1016/j.foodres.2015.06.027 PMid: 28454966
Pothakos V, De Vuyst L, Zhang SJ, et al. Temporal shotgun metagenomics of an Ecuadorian coffee fermentation process highlights the predominance of lactic acid bacteria. Curr Res Biotechnol 2020;2:1–15. https://doi.org/10.1016/j.crbiot.2020.02.001
Ribeiro LS, Miguel MGCP, Martinez SJ, et al. The use of mesophilic and lactic acid bacteria strains as starter cultures for improvement of coffee beans wet fermentation. World Journal of Microbiology and Biotechnology 2020;36:186. https://doi.org/10.1007/s11274-020-02963-7 PMid: 33219454
Abedi E, Hashemi SMB. Lactic acid production – producing microorganisms and substrates sources-state of art. Heliyon 2020;6(10):e04974. https://doi.org/10.1016/j.heliyon.2020.e04974 PMid: 33088933
Pereira GVM, Vale AS, Carvalho Neto DP, et al. Lactic acid bacteria: What coffee industry should know? Curr Opin Food Sci 2020;31:1–8. https://doi.org/10.1016/j.cofs.2019.07.004
Elhalis H, Cox J, Frank D, et al. The crucial role of yeasts in the wet fermentation of coffee beans and quality. Int J Food Microbiol 2020;333:108796. https://doi.org/10.1016/j.ijfoodmicro.2020.108796 PMid: 32771820
Swiegers JH, Bartowsky EJ, Henschke PA, et al. Yeast and bacterial modulation of wine aroma and flavour. Aust J Grape Wine Res 2005;11(2):139–173. https://doi.org/10.1111/j.1755-0238.2005.tb00285.x
Smarrito-Menozzi C, Matthey-Doret W, Devaud-Goumoens S, et al. Glycerol, an underestimated flavor precursor in the Maillard reaction. J Agric Food Chem 2013;61(43):10225–10230. https://doi.org/10.1021/jf3050044 PMid: 23373461
Martinez SJ, Rabelo MHS, Bressani APP, et al. Novel stainless steel tanks enhances coffee fermentation quality. Food Research International 2021;139:109921. https://doi.org/10.1016/j.foodres.2020.109921 PMid: 33509488
Martínez VMC, Aristizábal IDT, Moreno ELC. Evaluation of the composition effect of harvested coffee in the organoleptic properties of coffee drink. Vitae 2017;24:47–58. https://doi.org/10.17533/udea.vitae.v24n1a06

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyright (c) 2024 Electronic Journal of Biotechnology
