Effects of Agaricus blazei acidic polysaccharide on the aging of mice through keap1-Nrf2/ARE and MAPKs signal pathway
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Keywords

Acidic polysaccharide
Agaricus blazei Murrill
Aging
Anti-aging drugs
Edible fungus
Keap1-Nrf2/ ARE
Learning and memory
MAPKs
Mice

How to Cite

1.
Guo X, Ye Y, Liu X, Sheng Y, Yu Y, Yang Y, Gu M, Lin R, Wang B, An L, Lu X. Effects of Agaricus blazei acidic polysaccharide on the aging of mice through keap1-Nrf2/ARE and MAPKs signal pathway. Electron. J. Biotechnol. [Internet]. 2022 Jun. 16 [cited 2024 Oct. 9];57. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2022.03.004

Abstract

Background: In view of the increasing human life and the aging of the population, the search for safe anti-aging drugs has become a hot topic. Agaricus blazei Murrill is a rare edible fungus, with a variety of biological activities. The purpose of this study was to clarify the anti-aging effect and mechanism of ABM-A on the aging induced by D-Galactose in mice.

Results: The result showed that ABM-A contained 87.2% of glucose, 3.3% of galactose, 3.8% mannose and 5.7% gluconic acid. The behavior of mice in the treatment group was significantly improved after administration of ABM-A. And the activity of SOD and CAT and the level of T-AOC were increased (p < 0.05), the content of MDA and ROS was decreased (p < 0.05) in the serum of mice in ABM-A group. The results of mechanism research showed that nine genes were screened out by functional annotation and enrichment analysis for the verification by RT-qPCR, and the results of RT-qPCR were consistent with those of RNA-seq. Western Blot results showed that ABM-A upregulated the expression of Hmox1, Myd88, p-c-Jun, Apc, Bmil, Cox7a2l and Ndufv1, down-regulated the expression of Nfe2l2, Keap1, Apoe, Mapk1and Atp1a3, and decreased the phosphorylation of p38 and Jnk, suggesting that it may play an anti-aging effect by regulating Nrf2/ARE and MAPKs superfamily signal pathways.

Conclusions: ABM-A can reduce oxidation reaction and play an anti-aging role through Keap1-Nrf2/ARE and MAPKs signaling pathway.

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