Allitol bioproduction by recombinant Escherichia coli with NADH regeneration system co-expressing ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) in individual or in fusion
PDF

Keywords

Allitol
Bioproduction
Co-expression
Cofactor recycle
d-Allulose
Formate dehydrogenase
Fusion expression
NADH regeneration system
Recombinant Escherichia coli
Ribitol dehydrogenase
Sugar alcohol

How to Cite

1.
Wen X, Lin H, Ren Y, Li C, Zhang C, Lin J, Lin J. Allitol bioproduction by recombinant Escherichia coli with NADH regeneration system co-expressing ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) in individual or in fusion. Electron. J. Biotechnol. [Internet]. 2022 Jan. 4 [cited 2024 Nov. 11];55. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2021.11.007

Abstract

Background: As a kind of rare sugar alcohol, allitol has important application values in food and medication. In addition, it can be used as a key substrate to produce other d/l-rare sugars. Allitol can be effectively produced by the resting-cell biotransformation method.

Results: Two recombinant Escherichia coli strains, one simultaneously expressing ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) in fusion (fusion expression strain for short) and the other expressing the above two enzymes individually (individual expression strain for short), were respectively constructed and used for allitol bioproduction. The produced allitol was confirmed by HPLC, mass spectrometry, and polarimetry. The individual expression strain had higher activity, which produced 58.5 g/L allitol from 90 g/L d-allulose (also named d-psicose) in 1 h with an allitol productivity of 58.5 g/L/h under optimized conditions.

Conclusions: The constructed individual expression strain had the highest allitol productivity among the reports. The production process developed in this study was simple, highly efficient, and had the potential for mass production of allitol.

PDF

Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".

The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.