Systematic engineering of the rate-limiting step of β-alanine biosynthesis in Escherichia coli
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Keywords

Rate-limiting step
Thermoinduction
Two-stage fermentation

How to Cite

1.
Xu J, Zhu Y, Zhou Z. Systematic engineering of the rate-limiting step of β-alanine biosynthesis in Escherichia coli. Electron. J. Biotechnol. [Internet]. 2021 Jun. 9 [cited 2024 Nov. 2];51. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2021.03.002

Abstract

Background: Large amounts of β-alanine are required in fine chemical and pharmaceutical synthesis and other fields. Profitable and green methods are required for the industrial production of β-alanine.

Results: Replacing endogenous panD of Escherichia coli with heterologous CgpanD from Corynebacterium glutamicum enabled β-alanine synthesis of 0.67 g/L by strain B0016-082BB. Overexpressing CgpanD on both plasmids and chromosomes to enhance the rate-limiting step improved the β-alanine titer to 4.25 g/L in strain B0016-083BB/pPL451-panD with a slighter metabolic burden. Growth factors were introduced by addition of yeast extract, and 6.65 g/L of β-alanine was synthesized by strain B0016-083BB/pPL451-panD in the M9-3Y medium.

Conclusions: Enhancement of the rate-limiting steps in the β-alanine biosynthetic pathway, recruitment of the temperature-sensitive inducible pL promoter, and optimization of the fermentation process could efficiently increase β-alanine production in E. coli.

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