Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1
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Keywords

Alanine fermentation
Alanine mother liquor
DHA
Glucose
Growth factors
Growth
Industrial waste
Nitrogen source
Response surface methodology Schizochytrium sp. B4D1
Yeast

How to Cite

1.
Zhu Y, Li H, Chen L, Chen W, Cui M, Han L, Hou W, Li D. Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1. Electron. J. Biotechnol. [Internet]. 2018 Sep. 14 [cited 2024 Oct. 8];35. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2018.06.002

Abstract

Alanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surface methodology as follows: 99.98-g/L glucose, 0.05-g/L yeast extract and a 183.17 dilution factor of the alanine mother liquid (v/v, with an alanine content of 0.72 g/L) and 17.98% inoculum concentration (v/v). Finally, in a 50-mL shake-flask fermentation, the DHA yield was 2.29 g/L.

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