Effect of miR-125b on dermal papilla cells of goat secondary hair follicle
Reprint PDF

Keywords

anagen
Antagomir
cashmere goats
catagen
dermal papilla cells
hair cycle
hair follicle development
hair follicle morphogenesis
Luciferase assay
microRNAs
Recombinant adenovirus
telogen

How to Cite

1.
Zhou G, Yuan C, He X, Kang D, Wang X, Chen Y. Effect of miR-125b on dermal papilla cells of goat secondary hair follicle. Electron. J. Biotechnol. [Internet]. 2017 Jan. 12 [cited 2024 Dec. 8];25. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2016.11.006

Abstract

Background: MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes. miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and development. We identified that the expression of miR-125b varies during an individual hair cycle (anagen, catagen, and telogen) in the skin of cashmere goats. We constructed a gain model (by overexpressing miR-125b) and a loss model (by inhibiting endogenous miR-125b) based on dermal papilla cells (DPCs) to further investigate the role of miR-125b in HF cycle. In addition, we used a dual-luciferase system to highlight the predicated target genes of miR-125b.

Results: We found that miR-125b affects the expression of FGF5, IGF-1, SHH, TNF-α, MSX2, LEF-1, FGF7, NOGGIN, BMP2, BMP4, TGF-β1, and β-catenin. The dual-luciferase assay further validated a direct interaction between miR-125b and FGF5 and TNF-α.

Conclusion: miR-125b affects the expression levels of genes related to hair cycle and may also play a critical role in regulating the periodic development of HF.

Reprint PDF

Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".

The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.