Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from Amazon liquens
Reprint PDF

Keywords

factorial design
fibrinolytic activity
microbial enzymes
protease production
Streptomyces

How to Cite

1.
Silva GM, Bezerra RP, Teixeira JA, Porto TS, Lima-Filho JL, Porto AL. Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from Amazon liquens. Electron. J. Biotechnol. [Internet]. 2015 Jan. 19 [cited 2024 Dec. 8];18(1). Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2014.11.001

Abstract

Background: Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations.

Results: The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5mm2 was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions.

Conclusions: These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production.
Reprint PDF

Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".

The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.