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Vol 8, No 3 (2005)
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High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica | Radzi | Electronic Journal of Biotechnology
doi: 10.2225/vol8-issue3-fulltext-4
Electronic Journal of Biotechnology, Vol 8, No 3 (2005)

High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

Salina Mat Radzi, Mahiran Basri, Abu Bakar Salleh, Arbakariya Ariff, Rosfarizan Mohammad, Mohd. Basyaruddin Abdul Rahman, Raja Noor Zaliha Raja Abdul Rahman



Abstract

High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.




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ISSN:  0717-3458

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