doi: 10.2225/vol5-issue3-fulltext-5
Electronic Journal of Biotechnology, Vol 5, No 3 (2002)
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
Abstract
The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.