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Vol 13, No 5 (2010)
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Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut | Tang Wang | Electronic Journal of Biotechnology
doi: 10.2225/vol13-issue5-fulltext-18
Electronic Journal of Biotechnology, Vol 13, No 5 (2010)

Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut

Chuan Tang Wang, Shan Lin Yu, Shu Wei Zhang, Xiu Zhen Wang, Yue Yi Tang, Jian Cheng Zhang, Dian Xu Chen



Abstract

A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.




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ISSN:  0717-3458

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