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Vol 13, No 1 (2010)
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Genetic diversity of breeding popcorn lines determined by SSR markers | Ribeiro Trindade | Electronic Journal of Biotechnology
doi: 10.2225/vol13-issue1-fulltext-11
Electronic Journal of Biotechnology, Vol 13, No 1 (2010)

Genetic diversity of breeding popcorn lines determined by SSR markers

Ana Paula Ribeiro Trindade, Ronald José Barth Pinto, Antonio Teixeira do Amaral Júnior, Claudete Aparecida Mangolin, Maria de Fátima Pires da Silva Machado, Carlos Alberto Scapim



Abstract

Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations. Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers. Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification. The genetic distance was estimated by Rogers' modified distance. The different popcorn breeding programs in Brazil are possibly using highly similar base-populations. The genetic similarity of lines P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar. The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was reliable to discriminate the genotypes in five groups. The clusters were consistent with the estimates of genetic identity. There was a moderate coincidence degree between the groups and genealogy of lines. Higher levels of heterozygosity are expected from crosses between the group containing lines P3-3 and P7-3 with that of P1-3 and P7-4. Crosses between lines P1-3 and P8-1 are also promising.




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ISSN:  0717-3458

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