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Vol 12, No 2 (2009)
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Measuring β-Galactosidase activity at pH 6 with a differential pH sensor | Acevedo | Electronic Journal of Biotechnology
doi: 10.2225/vol12-issue2-fulltext-10
Electronic Journal of Biotechnology, Vol 12, No 2 (2009)

Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian A. Acevedo, Matthias H. Stach, Anette Amtmann, Manuel E. Young, Juan G. Reyes, Holger Huebner, Rainer Buchholz



Abstract

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.




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ISSN:  0717-3458

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