doi: 10.2225/vol12-issue2-fulltext-10
Electronic Journal of Biotechnology, Vol 12, No 2 (2009)
Measuring β-Galactosidase activity at pH 6 with a differential pH sensor
Abstract
The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.