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Vol 11, No 1 (2008)
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Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.) | Godoy-Hernández | Electronic Journal of Biotechnology
doi: 10.2225/vol11-issue1-fulltext-3
Electronic Journal of Biotechnology, Vol 11, No 1 (2008)

Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Gregorio Godoy-Hernández, Elidé Avilés-Berzunza, Mildred Carrillo-Pech, Felipe A. Vázquez-Flota



Abstract

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.




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ISSN:  0717-3458

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