• Log In
  • New issue alert
  • Submit a manuscript
  • Register
  • Home
  • About
  • Editorial Board
  • Search
  • Archives
  • Current
  • Forthcoming

Share

Article Panel


Vol 6, No 2 (2003)
»Table of Contents
Reading Tools
  • About the author
  • How to cite this article
  • Indexing metadata
  • Print version
  • Look up terms
  • Finding References
  • Review policy

Related items
  • Author's work


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International.
Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta | Velankar | Electronic Journal of Biotechnology
doi: 10.2225/vol6-issue2-fulltext-2
Electronic Journal of Biotechnology, Vol 6, No 2 (2003)

Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta

Harshad Ravindra Velankar, Mohan Ram Heble



Abstract

This paper reports biotransformation of (L)-citronellal to (L)-citronellol using free and immobilized cells of Rhodotorula minuta. The culture preparation variables such as pH, temperature and incubation period for obtaining maximum cell growth of R. minuta were optimized. The optimized culture conditions for free and immobilized cells of R. minuta. have been compared for (L)-citronellal biotransformation. The various factors such as the optimum substrate concentration and the time of substrate addition at varying cell concentrations during the growth of yeast culture were also studied. Highest (L)-citronellol concentration of 3.5 gl-1 was obtained with free cell catalyzed biotransformation at pH 5.5, 27ºC and 150 rpm after 8 hrs using initial (L)-citronellal concentration of 4.47 gl-1. Alginate immobilized R. minuta cells could optimally biotransform similar substrate concentration to 3.3 gl-1 (L)-citronellol at pH 6, 27ºC and 150 rpm after 8 hrs. Immobilized cells could be reused twice after the first run and the product concentrations of 2.63 gl-1 and 1.52 gl-1 were obtained during the first and the second reuse.




Full Text: | Full Text | Reprint PDF |

ISSN:  0717-3458

Contact: edbiotec@pucv.cl

Pontificia Universidad Católica de Valparaíso
Av. Brasil 2950, Valparaíso, Chile
Copyright © 1997- 2023 by Electronic Journal of Biotechnology