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Vol 35 (2018)
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Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1 | Zhu | Electronic Journal of Biotechnology
doi:10.1016/j.ejbt.2018.06.002
Electronic Journal of Biotechnology, Vol 35 (2018)

Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1

Yujing Zhu, Hanchen Li, Limei Chen, Wuxi Chen, Min Cui, Lina Han, Wenbo Hou, Demao Li



Abstract

Alanine mother liquor, a type of industrial waste from alanine fermentation, was used as a nitrogen source to produce docosahexaenoic acid (DHA) by Schizochytrium sp. B4D1. The results indicated that yeast extract could trigger the utilization of the alanine mother liquor. Additionally, the alanine can be quenched during the culture, which aids in DHA accumulation. The medium components were optimized via response surface methodology as follows: 99.98-g/L glucose, 0.05-g/L yeast extract and a 183.17 dilution factor of the alanine mother liquid (v/v, with an alanine content of 0.72 g/L) and 17.98% inoculum concentration (v/v). Finally, in a 50-mL shake-flask fermentation, the DHA yield was 2.29 g/L.




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ISSN:  0717-3458

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