• Log In
  • New issue alert
  • Submit a manuscript
  • Register
  • Home
  • About
  • Editorial Board
  • Search
  • Archives
  • Current
  • Forthcoming

Share

Article Panel


Vol 28 (2017)
»Table of Contents
Reading Tools
  • About the author
  • How to cite this article
  • Indexing metadata
  • Print version
  • Look up terms
  • Finding References
  • Review policy

Related items
  • Author's work


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International.
Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing | Yan | Electronic Journal of Biotechnology
doi:10.1016/j.ejbt.2017.05.006
Electronic Journal of Biotechnology, Vol 28 (2017)

Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing

Kuan Yan, Qin Wei, Ruizhang Feng, Wanhai Zhou, Fang Chen



Abstract

Background: Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood due to the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis.

Results: Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using Trinity and yielded 82,061 unigenes with an average length of 879.43bp and N50 value of 1,387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis indicated that our assembly is 91% complete. The unigenes were used to query the non-redundant database based on sequence similarity; 42,809 unigenes were homologous to known genes indifferent species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Additionally, 23,463 simple sequence repeats were identified using the microsatellite identification tool.

Conclusion: This is the first detailed transcriptome analysis of C. longepaniculatum. The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum.




Full Text: | PDF | HTML

ISSN:  0717-3458

Contact: edbiotec@pucv.cl

Pontificia Universidad Católica de Valparaíso
Av. Brasil 2950, Valparaíso, Chile
Copyright © 1997- 2022 by Electronic Journal of Biotechnology