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Vol 17, No 1 (2014)
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Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 | Rufino | Electronic Journal of Biotechnology
doi:10.1016/j.ejbt.2013.12.006
Electronic Journal of Biotechnology, Vol 17, No 1 (2014)

Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988

Raquel Diniz Rufino, Juliana Moura Luna, Galba Maria Campos Takaki, Leonie Asfora Sarubbo



Abstract

Background: The biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established.

Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of carbohydrates.

Conclusions: The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea, Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant produced suggest its potential application in industries that require the use of effective compounds at low cost.

© 2014 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserved.




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ISSN:  0717-3458

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