A new method for fast determination of biocatalysts process stability M. Boy* A Dominik,
H. Voss * Corresponding
author
A major bottleneck in developing industrial biocatalysis is the lack of a method to determine the catalyst process stability quickly and efficiently. In particular, the determination of these properties is difficult, often inaccurate and expensive because highly active, selective and stable biocatalysts should be achieved. In order to solve this problem, a new method for fast in-situ-characterization of activity and long-term stability of enzyme carrier catalysts has been developed. To accelerate the process of ageing the temperature is increased continuously during the experiment. On the basis of the proposed dynamic model it is possible to determine information pertaining to enzyme kinetics, selectivity, interaction between carrier, enzyme and substrate as well as information regarding the stability of the enzyme carrier complex, including reversible and irreversible activation and inactivation parameters. In contrast to common, isothermal techniques these parameters can be estimated for the whole temperature range in one short experiment. Working under industrial proces conditions allows one to determine the whole relevant information necessary to characterise the (carrier-bound) biocatalyst. This knowledge allows the design and optimisation of biocatalytic industrial processes within a short period of time. |
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