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Table 2. 25-1 fractional factorial experiment for supplement screening. Cells were inoculated in different media at a final concentration of 2.5 x 105 cells.ml-1 and cultivated at 28ºC. |
|
Assay |
Independent Variables |
Response Variables |
|||||||
|
|
GLUa (g.l-1) |
YEb (g.l-1) |
PF68c (%) |
FBSd (%) |
MWRe (%) |
Xsf (x105 viable cells.ml-1) |
Xmeg (x105 viable cells.ml-1) |
Vmeh (%) |
µi (h-1) |
|
1A |
1.8 |
0.0 |
0.0 |
1.0 |
5.0 |
21.3 |
9.6 |
99 |
0.0230 |
|
2A |
2.7 |
0.0 |
0.0 |
1.0 |
0.0 |
2.1 |
1.7 |
75 |
0.0064 |
|
3A |
1.8 |
8.0 |
0.0 |
1.0 |
0.0 |
40.4 |
14.9 |
90 |
0.0141 |
|
4A |
2.7 |
8.0 |
0.0 |
1.0 |
5.0 |
44.1 |
10.2 |
93 |
0.0223 |
|
5A |
1.8 |
0.0 |
0.2 |
1.0 |
0.0 |
3.3 |
2.5 |
84 |
0.0069 |
|
6A |
2.7 |
0.0 |
0.2 |
1.0 |
5.0 |
20.7 |
7.8 |
95 |
0.0223 |
|
7A |
1.8 |
8.0 |
0.2 |
1.0 |
5.0 |
35.4 |
10.7 |
91 |
0.0247 |
|
8A |
2.7 |
8.0 |
0.2 |
1.0 |
0.0 |
39.6 |
8.5 |
96 |
0.0223 |
|
9A |
1.8 |
0.0 |
0.0 |
3.0 |
0.0 |
5.0 |
4.1 |
99 |
0.0223 |
|
10A |
2.7 |
0.0 |
0.0 |
3.0 |
5.0 |
19.2 |
9.4 |
98 |
0.0231 |
|
11A |
1.8 |
8.0 |
0.0 |
3.0 |
5.0 |
41.8 |
17.0 |
99 |
0.0165 |
|
12A |
2.7 |
8.0 |
0.0 |
3.0 |
0.0 |
36.5 |
13.0 |
92 |
0.0161 |
|
13A |
1.8 |
0.0 |
0.2 |
3.0 |
5.0 |
23.2 |
9.6 |
98 |
0.0187 |
|
14A |
2.7 |
0.0 |
0.2 |
3.0 |
0.0 |
6.4 |
4.3 |
86 |
0.0167 |
|
15A |
1.8 |
8.0 |
0.2 |
3.0 |
0.0 |
36.6 |
8.4 |
87 |
0.0187 |
|
16A |
2.7 |
8.0 |
0.2 |
3.0 |
5.0 |
47.0 |
15.8 |
98 |
0.0239 |
|
Grace's+FBS |
0.7 |
- |
- |
10.0 |
0.0 |
9.0 |
4.0 |
80 |
0.0266 |
Data presents the ranges of the independent variables aglucose,byeast extract, cPluronic F68, dfetal bovine serum, and emilk whey retentate on the dependent variables fviable cell concentration in stationary phase, gviable cell concentration in midexponential phase, hviability in midexponential phase and ispecific cell growth rate. |
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