Table 1 - Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment

Table 1. Summary of treatments subjected to leaf segments of Nicotiana tabacum prior to co-cultivation with A. rhizogenes.

Treatment	Sequence of treatments prior to co-cultivation or during co-cultivation
A	Manual wounding
B	Ultra-sonication
C1	Ultra-sonication + Pectinase^a 0.1%
C2	Ultra-sonication + Pectinase^a 0.5%
C3	Ultra-sonication + Pectinase^a 1%
D1	Ultra-sonication + Cellulase^a 0.1%
D2	Ultra-sonication + Cellulase^a 0.5%
D3	Ultra-sonication + Cellulase^a 1%
E1	Ultra-sonication + Pectinase and Cellulase^a 0.1%
E2	Ultra-sonication + Pectinase and Cellulase^a 0.5%
E3	Ultra-sonication + Pectinase and Cellulase^a 1%
F1	Ultra-sonication + acetosyringone^b 50 µM
F2	Ultra-sonication + acetosyringone^b 100 µM
F3	Ultra-sonication + acetosyringone^b 150 µM
G1	Ultra-sonication + CaCl₂^b5 mM
G2	Ultra-sonication + CaCl₂^b 10 mM
G3	Ultra-sonication + CaCl₂^b 20 mM

^a Prior to co-cultivation with A. rhizogenes.
^bDuring co-cultivation with A. rhizogenes.