|
|
Table 1. Determination of PAb-Mn SOD in
resistant and susceptible pearl millet genotype after inoculation with
S. graminicola. Two-day-old resistant (IP 18293) and susceptible
(23B) pearl millet seedlings were inoculated with zoospore suspension
of S. graminicola (40 x 104 zoospores/ml). Seedlings
harvested at regular intervals of two hours up to 24 hrs were used for
the enzyme extraction. Protein (50 μg) were loaded to the wells
of ELISA plate. Primary antibody was used at a dilution of 1:20,000.
The reaction was read at 410 nm using microtitre plate reader. Data
are mean (± SD) of three independent experiments with reading determined
in triplicates. |
|
Time after inoculation |
Absorbance at 410 nm |
|||
|
Resistant |
Susceptible |
|||
|
Uninoculated |
Inoculated |
Uninoculated |
Inoculated |
|
| 0 |
0.22 ± 0.017 |
0.22 ± 0.019 |
0.24 ± 0.0204 |
0.23 ± 0.016 |
| 2 |
0.26 ± 0.018 |
0.27 ± 0.018 |
0.29 ± 0.0168 |
0.24 ± 0.024 |
| 4 |
0.28 ± 0.041 |
0.51 ± 0.018 |
0.38 ± 0.0246 |
0.30 ± 0.018 |
| 6 |
0.33 ± 0.031 |
0.40 ± 0.016 |
0.32 ± 0.019 |
0.29 ± 0.031 |
| 8 |
0.30 ± 0.037 |
0.37 ± 0.041 |
0.28 ± 0.014 |
0.27 ± 0.017 |
| 10 |
0.27 ± 0.034 |
0.35 ± 0.032 |
0.24 ± 0.0179 |
0.23 ± 0.018 |
| 12 |
0.24 ± 0.024 |
0.31 ± 0.029 |
0.22 ± 0.0181 |
0.23 ± 0.031 |
| 14 |
0.24 ± 0.025 |
0.27 ± 0.028 |
0.23 ± 0.0163 |
0.23 ± 0.018 |
| 16 |
0.24 ± 0.035 |
0.27 ± 0.021 |
0.23 ± 0.028 |
0.24 ± 0.02 |
| 18 |
0.23 ± 0.031 |
0.27 ± 0.023 |
0.23 ± 0.021 |
0.25 ± 0.017 |
| 20 |
0.23 ± 0.016 |
0.26 ± 0.026 |
0.23 ± 0.024 |
0.25 ± 0.018 |
| 22 |
0.22 ± 0.021 |
0.25 ± 0.015 |
0.23 ± 0.013 |
0.24 ± 0.016 |
| 24 |
0.22 ± 0.025 |
0.24 ± 0.016 |
0.23 ± 0.015 |
0.24 ± 0.041 |
|
|