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Table 1. Primers used in this study. |
|
Primers |
Sequences |
Applications |
| P1 | 5-CCCAAGCTTGAATGCTTGTATTGACC | To clone the upstream sequence |
| P2 | 5-GCGTCGACGGATTTATCTCCTCTTG | |
| P3 | 5-ATGGTGAGCAAGGGCGAG | To delete the additional sequence |
| P4 | 5-GGATTTATCTCCTACTTG | |
| P5 | 5-CCTGCGTTATCCCCTGATTCTGTGG | To select the deletion mutants |
| P6 | 5-GAACAGCTCCTCGCCCTTGCTCACC | |
| P7 | 5-CATGCAGACTTACAACAACCC | To clone the isiAB gene fragment |
| P8 | 5-AGCCAACTGTTTGACTACC | |
| PdL | 5-TCAATACAAGCATTCAAG | Paired with the below primers to produce a series of 5' end deletion mutations of the upstream sequence of cpcB gene
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| PdR1 | 5-AGAAGGAAGGCGAATGTTG | |
| PdR2 | 5-ATTCTTCTCATAAACCCTG | |
| PdR3 | 5-AGCCTTTGTCGATGGTTCG | |
| PdR4 | 5-ACCGATGGATTGATTGTCG | |
|
PdR5 |
5-CCCAACTCAACTCTAAGC |
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