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Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides Ana
Cláudia Santana de Almeida Luciares
Costa de Araújo Andressa
Mendes Costa César
Augusto Moraes de Abreu Maria
Alice Gomes de Andrade Lima Maria de Los
Angeles Perez Fernandez Palha* *Corresponding author
Financial support: CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).
The enzyme known
as invertase (E.C. 3.2.1.26 - β-D-fructofuranosidase)
catalyzes the sucrose hydrolysis producing an equimolar mixture of
glucose and fructose named inverted sugar. The fungus Cladosporium
cladosporioides has invertase as its constituent. Hence, its use
as a natural immobilized support for the invertase produces interesting
results for the enzyme. The present work has the objective of determining
the optimum operational conditions of auto-immobilized invertase,
as well as its kinetic parameters (KM and Vmax).
A complete 23 factorial planning was done for the evaluation
of such parameters. Temperature, pH and agitation level were the studied
variables. The hydrolysis percentage was the monitored result. Batch
tests in optimum conditions were done to determine the kinetic parameters.
Temperature of |
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