Figure 3. Proteolytic degradation of MB-1TrpHis and mutants by E. coli proteases. At variance with results in Figure 2, proteolysis was performed after separating MB-1TrpHis mutants from its fusion partner MBP.

Symbols: MB-1TrpHis; E44V45; E44M45.

The average standard deviation for undegraded protein measurements is 5%.