Figure 6. Effect of organic solvents on the transfructosylation reaction of LsdA. Sucrose at concentrations of 100 mM A) and 580 mM B) was incubated for 10 hours in buffer NaAc 100 mM, pH 5.5, in the presence of 10 IU of LsdA expressed by P. pastoris (Pp), natural host G. diazotrophicus (Gd) or recombinant E. coli strain (Ec) and 50% (V/V) of Acetone (Ac), Acetonitrile (An) or DMSO (D) at the indicated temperature. Product profiles were analyzed by TLC.

Symbols represent: (F) fructose, (S) sucrose, (K) kestose, (L) levan and (M) sugars marker.