|
| Table
1. Mutagenesis
and selection of mutants. |
|
Mutagenesis conditions |
Colonies |
Transfers |
Stability |
|
|
Concentration of MNNG*, μg/ml |
Exposure time, min |
|||
| 100 (OD=1.2) |
20 |
3 |
2 |
- |
| 30 |
none |
|||
| 40 |
none |
|||
| 75 (OD=1.2) |
30 |
11 |
3 |
- |
| 50 (OD=0.684) |
30 |
19 |
6 |
+ |
| 50 (OD=0.81) |
30 |
14 |
6 |
+ |
| 50 (OD=1.24) |
30 |
3 |
6 |
+ |
| MNNG:N-methyl,
N'-nitro, N-nitrosoguanidine |
| |