Plant Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 6 No. 3, Issue of December 15, 2003
© 2003 by Pontificia Universidad Católica de Valparaíso -- Chile Received March 6, 2003 / Accepted December 4, 2003

Root induction in radiata pine using Agrobacterium rhizogenes

Mingshan Li
Department of Biochemistry
University of Missouri-Columbia
Columbia, MO 65211, USA
Tel: 573 884 6334

David W.M. Leung*
School of Biological Sciences
University of Canterbury
Private Bag 4800, Christchurch, New Zealand
Tel: 64 3 364 2650
Fax: 64 3 364 2590

*Corresponding author

Financial support: Doctoral scholarship from the University of Canterbury to Mingshan Li.

Keywords: Agrobacterium rhizogenes, auxin, co-cultivation, Pinus radiate, rooting.

Full Text

Root induction using Agrobacterium rhizogenes was conducted in hypocotyl explants, intact seedlings, de-rooted seedling cuttings and adventitious shoots of radiata pine (Pinus radiata D. Don). Use of two A. rhizogenes strains (A4T and LB9402), with or without application of IBA, can trigger root formation in different explants. Strain LBA9402 was more effective than A4T in increasing rooting percentage and root number. Addition of 4.4 uMIBA to the medium further enhanced rooting from the cultured hypocotyl segments inoculated with the two A. rhizogenes strains. Strain LBA9402+IBA induced in about 75% of the cultured hypocotyl segments to form roots. In contrast the controls failed to initiate roots on intact seedlings or cultured segments in presence or absence of IBA. Rooting of adventitious shoots from 3 year-old radiata pine was improved following inoculation with LBA9402, suggesting that this rooting treatment has potential to aid clonal propagation of radiata pine.

Supported by UNESCO / MIRCEN network 
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