Process Biotechnology

Biotechnology Industry

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 6 No. 3, Issue of December 15, 2003
© 2003 by Pontificia Universidad Católica de Valparaíso -- Chile Received May 2, 2003 / Accepted November 10, 2003
REVIEW ARTICLE

Protein interactions in enzymatic processes in textiles

Tzanko Tzanov
Department of Textile Engineering
University of Minho
4800 Guimaraes, Portugal
Tel: 34 9373 98149
Fax: 34 9373 98101
E-mail: tzanko.tzanov@upc.es

Jürgen Andreaus
Department of Chemistry
Universidade Regional de Blumenau
89010-971, Blumenau, Brazil
Tel: 55 47 3210468 / 276
Fax: 55 47 3228818 / 3210232
E-mail: jandr@furb.br

Georg Guebitz
Institute of Environmental Biotechnology
TU- Graz , A-8010 Graz, Austria
Tel: 43 316 8738312
Fax: 43 316 8738815
E-mail: guebitz@ima.tu-graz.ac.at

Artur Cavaco-Paulo*
Department of Textile Engineering
University of Minho
4800 Guimaraes, Portugal
Tel: 351 253 510280
Fax. 351 253 510293
E-mail: artur@det.uminho.pt

*Corresponding author

Keywords: auxiliaries, dyes, protein interactions, surfactants, textile processing.

Abstract Full Text

Enzymes are the catalysts of all reactions in living systems. These reactions are catalysed in the active sites of globular proteins. The proteins are composed by amino acids with a variety of side chains ranging from non-polar aliphatic and aromatic to acidic, basic and neutral polar. This fact allows to a globular 3D protein to create in the active site all ranges of microenvironments for catalysis. Major advances in microbial technology and genetics allow recently the broad range of enzymatic applications in the industry. Enzymatic processes have been increasingly incorporated in textiles over the last years. Cotton, wool, flax or starches are natural materials used in textiles that can be processed with enzymes. Enzymes have been used for desizing, scouring, polishing, washing, degumming, peroxide degradation in bleaching baths as well as for decolourisation of dyehouse wastewaters, bleaching of released dyestuff and inhibiting dye transfer. Furthermore many new applications are under development such as natural and synthetic fibres modification, enzymatic dyeing, finishing etc. Most of the textile processes are heterogeneous where an auxiliary as a dye, enzyme, softener or oxidant have to be taken from the solution to the fibre. These processes require the presence of surface-active agents, ionic force "balancers", buffers, stabilisers and others, and are characterized with high turbulence and mechanical agitation in the textile baths.  In this paper it is intended to understand and discuss the major protein interactions within textile processes and to try to anticipate troubleshooting possibilities when enzymes are used. It can be expected that an enzyme protein can interact with all chemical agents in solution due to the large variety of side chains of the outer-amino-acids in the large 3D structure of the protein. Without the aim of being exhaustive various points will be discussed where protein interactions are important for textile processing.

 
Supported by UNESCO / MIRCEN network