Molecular Biology and Genetics

Plant Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 6 No. 3, Issue of December 15, 2003
© 2003 by Pontificia Universidad Católica de Valparaíso -- Chile Received Febraury 21, 2003 / Accepted December 5, 2003
RESEARCH ARTICLE

Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers

Liliana Martínez*
Laboratorio de Biología Molecular
Departamento de Ciencias Biológicas
Facultad de Ciencias Agrarias
Universidad Nacional de Cuyo
Almirante Brown 500, Chacras de Coria, M5528AHB
Mendoza, Argentina
Tel: 0054 261 496004 (ext 2032)
Fax: 0054-261-4960469
E-mail: lmartinez@fca.uncu.edu.ar

Pablo Cavagnaro
Laboratorio de Biología Molecular
Departamento de Ciencias Biológicas
Facultad de Ciencias Agrarias
Universidad Nacional de Cuyo
Almirante Brown 500, Chacras de Coria, M5528AHB
Mendoza, Argentina
Tel: 0054 261 496004 (ext 2032)
Fax: 0054 261 4960469
E-mail: pfcavagnaro@hotmail.com

Ricardo Masuelli
Laboratorio de Biología Molecular
Departamento de Ciencias Biológicas
Facultad de Ciencias Agrarias
Universidad Nacional de Cuyo
Almirante Brown 500, Chacras de Coria, M5528AHB
Mendoza, Argentina
Tel: 0054 261 496004 (ext 2032)
Fax: 0054 261 4960469
E-mail: rmasuelli@fca.uncu.edu.ar

José Rodríguez
Cátedra de Viticultura
Departamento de Producción Agropecuaria
Facultad de Ciencias Agrarias
Universidad Nacional de Cuyo
Almirante Brown 500, Chacras de Coria, M5528AHB
Mendoza, Argentina
Tel: 0054 261 496004 (ext 2032)
Fax: 0054 261 4960469
E-mail: jrodriguez@fca.uncu.edu.ar

*Corresponding author


Financial support:
The present work has been done in the framework of a research programme (1998-2002) funded by SECYT (Secretaría de Ciencia y Técnica) of the National University of Cuyo.

Keywords: ampelography, Criollas, genetic variability, molecular markers.

Abbreviations:

AFLP: Amplified Fragment Length Polymorphism;
NTSYS:Numerical Taxonomy and Multivariate Analysis System;
O.I.V.: Office International du Vin;
PCR: Polymerase Chain Reaction;
RAPD: Random Amplified Polymorphic DNA;
RFLP: Restriction Fragment Length Polymorphism;
SSR: Simple Sequence Repeats.

Abstract
Full Text

Half of the Argentine grapevine growing area is cultivated with local varieties generically called "Criollas". These materials differ in morphology, physiology and enological aptitudes from traditional European varieties. To discriminate among them, we used morphological markers to evaluate the genetic diversity and phenetic relatedness of 9 Criollas, 6 European and 1 American varieties and compared with AFLP markers. Three AFLP primer combinations generated a total of 111 scorable fragments. Dendrograms obtained with morphologic and AFLP markers agreed in clustering the "Criollas" separately from the European and American varieties assayed, except for Muscat d' Alexandrie and Tempranillo which clustered with Criollas in the case of AFLP. Comparison of UPGMA dendrograms of morphological and AFLP markers using the Mantel test indicated a  not significant correlation of r = 0.33. Nevertheless, AFLP and selected morphological characters appear as useful and complementary techniques for grapevine identification and for evaluation of genetic diversity. Among the "Criollas", AFLP similarities ranged from 76 to 98% (Dice coefficient), indicating an important source of genetic diversity that can be exploited in future breeding programs. To our knowledge, this is the first report using AFLP markers to assess genetic variability on these materials.

 
Supported by UNESCO / MIRCEN network 
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