|
| Table 2. | Quantification
of rhGM-CSF in cell culture supernatants using different assays. |
| Assay |
rhGM-CSF concentration |
Specific Activity a |
| Sandwich ELISA with MAb-Polyclonal |
2.1 ng/ml |
1,094 U/ng |
| Sandwich ELISA with 2 Mabs |
2.6 ng/ml |
884 U/ng |
| Competitive ELISA |
422.0 ng/ml |
5 U/ng |
| In vitro biological activity |
2,297.2 U/ml |
------------- |
aThe
specific activity was estimated as the ratio between the in vitro
biological activity and the concentration estimated by ELISA.
|
|
|