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Molecular Biology and Genetics |
A
laboratory scale device for microencapsulation of genetically engineered cells
into alginate beads Gabriel
León Fiszman
Unidad de Transferencia
Genética,
Instituto de Oncología "A.H. Roffo" - UBA
Av. San
Martín 5481
1417 Buenos Aires
Argentina
Tel/fax: 54 11 4580 2813
E-mail: gfiszman@ciudad.com.ar Armando
Luis Karara
Unidad de Transferencia
Genética,
Instituto de Oncología "A.H. Roffo" - UBA
Av. San
Martín 5481
1417 Buenos Aires
ArgentinaTel/fax: 54 11 4580 2813
E-mail:
alkarara@hotmail.com Liliana
María Elena Finocchiaro+
Unidad
de Transferencia Genética,
Instituto de Oncología "A.H. Roffo" -
UBA
Av. San Martín 5481
1417 Buenos Aires
Argentina
Tel/fax: 54
11 4580 2813
E-mail: gglikin@bg.fcen.uba.ar Gerardo
Claudio Glikin*+
Unidad
de Transferencia Genética,
Instituto de Oncología "A.H. Roffo" -
UBA
Av. San Martín 5481
1417 Buenos Aires
Argentina
Tel/fax: 54
11 4580 2813
E-mail: gglikin@bg.fcen.uba.ar
* Corresponding author
Financial support:
This work was partially supported by grants from FONCYT and CABBIO. A.L.K;
G.C.G., L.M.E.F. are members of the Consejo Nacional de Investigaciones Científicas
y Técnicas (CONICET), Argentina. G.L.F. is a fellow of BioSidus S.A.
Keywords:
CHO cells, interleukin-2, protein delivery.
+On
leave of absence from INGEBI-CONICET/FCEN-UBA
The microencapsulation
of recombinant cells, widely used for in vitro high-density cell
culture, is a novel and potentially cost-effective method of in vivo
heterologous protein delivery, where the protein producing cells are
immunologically protected from tissue rejection. We
report here a simple, reliable and inexpensive laboratory method to
generate calcium alginate microcapsules containing genetically engineered,
interleukin-2 expressing, Chinese hamster ovary (CHO) cells.
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