|
| Table
4. Comparative study of L-DOPA production and kinetic parameters (volumetric rates)
by using parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. |
|
Incubation period (Hours) |
Volumetric rates (mg/ml/h) | |||||
| Qp |
Qs |
Qx | ||||
| Parent |
Mutant |
Parent |
Mutant |
Parent |
Mutant | |
| 24 36 48 60 72 84 96 |
0.008±0.002 0.009±0.001 0.007±0.001 0.005±0.001 0.004±0.003 0.003±0.001 0.002±0.001 |
0.007±0.001 0.019±0.001 0.028±0.001 0.021±0.002 0.012±0.002 0.011±0.001 0.014±0.001 |
0.020±0.002 0.017±0.002 0.018±0.002 0.018±0.002 0.019±0.002 0.021±0.002 0.020±0.003 |
0.011±0.002 0.013±0.002 0.015±0.001 0.013±0.001 0.012±0.001 0.011±0.002 0.014±0.001 |
0.0025±0.001 0.0022±0.001 0.002±0.002 0.0017±0.001 0.0017±0.0002 0.0017±0.0001 0.0001±0.0002 |
0.0017±0.0001 0.0025±0.0001 0.0029±0.0002 0.0023±0.0002 0.0018±0.0002 0.0015±0.0003 0.0017±0.0001 |
| Qp
= Slope of product (mg/ml) / time of fermentation (h); Qs = Slope of
sustrate utilized (mg/ml) / time of fermentation (h); Qx = Slope of
cell mass formation (mg/ml) / time of fermentation (h); ± indicates the standard
deviation of three parallel sets of replicates, within the column, values differ
significantly at p<0.005. |
| |