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Table 3. Biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae using shake flasks.
| Incubation
period |
L-DOPA produced (mg/ml) |
L-tyrosine used (mg/ml) |
% L-DOPA* |
Growth yield coefficient, Yx/s (mg/mg) | ||||
| Parent |
Mutant |
Parent |
Mutant |
Parent |
Mutant |
Parent |
Mutant | |
| 24 36 48 60 72 84 96 |
0.21 0.34 0.36 0.32 0.29 0.24 0.18 |
0.16 0.68 1.34 1.26 1.12 1.06 0.92 |
0.48 0.62 0.86 1.10 1.39 1.78 1.96 |
0.26 0.48 0.74 0.80 0.86 0.96 1.38 |
43.75 54.84 41.86 29.09 20.86 13.48 9.18 |
61.54 80.42 87.55 70.52 62.50 50.02 37.24 |
0.013±0.002 0.013±0.001 0.014±0.001 0.011±0.001 0.009±0.001 0.008±0.001 0.008±0.001 |
0.015±0.002 0.019±0.002 0.019±0.002 0.017±0.002 0.015±0.001 0.013±0.001 0.011±0.002 |
| L-tyrosine added 2.5 mg/ml, Incubation temperature 30șC, Agitation intensity 160rpm, *on the basis of L-tyrosine used. Yx/s = Dry cell mass (mg/ml) / Substrate utilized (mg/ml), ± indicates the standard deviation among the three parallel replicates, the values differ significantly at p<0.008. |
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