|
| Table
2. Comparative study of L-DOPA production and kinetic parameters of parental strain
of Aspergillus oryzae and its best UV-irradiated mutant strains. |
|
Kinetic parameters |
L-DOPA | |||
| Parent |
UV-3 |
UV-6 |
UV-7 | |
| µ (h-1) | 0.0067±0.02 |
0.0154±0.02 |
0.0170±0.02 |
0.0267±0.02 |
Yx/s
(mg/mg) | 0.0087±0.01 | 0.0087±0.02 | 0.0086±0.01 | 0.0081±0.03 |
Qs
(mg/ml/h) | 0.0192±0.02 | 0.0220±0.01 | 0.0196±0.02 | 0.0179±0.03 |
qs
(mg/mg/h) | 0.0062±0.02 | 0.0071±0.01 | 0.0062±0.01 | 0.0058±0.03 |
Yp/x
(mg/mg) | 40.000±0.01 | 92.500±0.01 | 102.50±0.01 | 182.86±0.02 |
Yp/s
(mg/mg) | 0.3478±0.02 | 0.6981±0.02 | 0.8723±0.02 | 1.4883±0.02 |
Qp
(mg/ml/h) | 0.0066±0.01 | 0.0150±0.02 | 0.0165±0.02 | 0.0266±0.02 |
qp
(mg/mg/h) | 0.0021±0.01 | 0.0050±0.01 | 0.0055±0.02 | 0.0086±0.02 |
| µ = specific growth rate (h-1); Yx/s = cell mass yield (mg/mg); Qs = slope of substrate utilized (mg/ml/h) / time of fermentation (h); qs = specific substrate uptake rate (mg/mg/h) = (µ x Yx/s); Yp/x = product (mg/ml) / cell mass (mg/ml); Yp/s = product (mg/ml) / substrate utilized (mg/ml); Qp = slope of product (mg/ml/h) / time of fermentation (h); qp = specific citric acid production rate (mg/mg/h) = (µ x Yp/s); ± indicates the standard deviation among the three parallel replicates. Within the rows, the values differ significantly at p<0.05. |
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