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Table 1. Screening of UV-irradiated mutant strains of Aspergillus oryzae for the production of L-DOPA in shake flasks.
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Mutant strains of Aspergillus oryzae |
L-tyrosine used (mg/ml) |
Dry cell mass (mg/ml) |
L-DOPA produced (mg/ml) |
Mycelial morphology |
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Parent (GCB-6) UV-1 UV-2 UV-3 UV-4 UV-5 UV-6 UV-7 UV-8 UV-9 UV-10 |
0.92 0.95 1.21 1.06 1.35 0.87 0.94 0.86 1.42 1.68 0.98 |
0.008 0.004 0.005 0.008 0.011 0.010 0.008 0.007 0.009 0.014 0.012 |
0.32 0.16 0.25 0.74 0.30 0.08 0.82 1.28 0.10 0.26 0.18 |
Fine round pellets Gelatinous mass " Small pellets Gelatinous mass " Small pellets Intermediate pellets Gelatinous mass " " |
| Incubation temperature 30ºC; Initial pH 5.5; Agitation intensity 160 rpm. |
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