Table 1. Screening of UV-irradiated mutant strains of Aspergillus oryzae for the production of L-DOPA in shake flasks.

Mutant strains of Aspergillus oryzae

L-tyrosine used (mg/ml)

Dry cell mass (mg/ml)

L-DOPA produced (mg/ml)

Mycelial morphology

Parent (GCB-6)

UV-1

UV-2

UV-3

UV-4

UV-5

UV-6

UV-7

UV-8

UV-9

UV-10

0.92

0.95

1.21

1.06

1.35

0.87

0.94

0.86

1.42

1.68

0.98

0.008

0.004

0.005

0.008

0.011

0.010

0.008

0.007

0.009

0.014

0.012

0.32

0.16

0.25

0.74

0.30

0.08

0.82

1.28

0.10

0.26

0.18

Fine round pellets

Gelatinous mass

"

Small pellets

Gelatinous mass

"

Small pellets

Intermediate pellets

Gelatinous mass

"

"

Incubation temperature 30ºC; Initial pH 5.5; Agitation intensity 160 rpm.

Supported by UNESCO / MIRCEN network