Table 1. dsDNase activity of 2-week-old rice cell extract in the presence of cations, EDTA and EGTA (n = 3 ± standard error).

dsDNase activity (dA260 mg of protein-1 min-1)

Ion/chelating agent

1 mM

10 mM

Control

0.056 ± 0.02

0.056 ± 0.02

Ca

0.037 ± 0.01

0.026 ± 0.02

Mg

0.043 ± 0.00

0.058 ± 0.01

Mn

0.06 ± 0.02

0.024 ± 0.01

Cu

0.018 ± 0.01

0.027 ± 0.01

Zn

0.063 ± 0.00

0.089 ± 0.01

EDTA

0.019 ± 0.00

0.056 ± 0.01

EGTA

0.053 ± 0.02

0.076 ± 0.02


Supported by UNESCO / MIRCEN network