|
|
Table 2. Determination
of purity of mitochondrial fractions, obtained from Saccharomyces cerevisiae
strains NBIMCC 582, NBIMCC 583 and NBIMCC 584 trough measurement specific
activities of cytosolic 1, peroxisomal 2 and mitochondrial
3 marker enzymes.
|
|
Strains
|
NBIMCC 582 |
NBIMCC 583 |
NBIMCC 584 |
NBIMCC 582 |
NBIMCC 583 |
NBIMCC 584 |
|||
|
Enzyme activities |
Mitochondrial fraction |
Cell free extract |
|||||||
|
Catalase |
5.3 + 0.3 |
6.8 + 0.2 |
5.5 + 0.1 |
6.6 + 0.3 |
10.9 + 0.4 |
7.34 + 0.3 |
|||
|
Peroxidase |
0.17 ± 0.01 |
0.18 ± 0.01 |
0.16 ± 0.02 |
0.02 ± 0.01 |
0.02 ± 0.012 |
0.03 ± 0.014 |
|||
|
Glucose-6 phosphate dehydrogenase1 |
None |
None |
None |
14.16 + 0.02 |
18.74 + 0.05 |
19.04 + 0.02 |
|||
|
Hexokinase1 |
None |
None |
None |
0.020 ± 0.010 |
0.025 ± 0.002 |
0.020 ± 0.015 |
|||
|
D aminoacid oxidase2 |
None |
None |
None |
0.058 ± 0.001 |
0,108 ± 0.001 |
0,076 ± 0.001 |
|||
|
Isocitic lyase2
[U/mg protein] |
None
|
None
|
None
|
0.102 + 0.013
|
0.098 + 0.023
|
0.108 + 0.015
|
|||
|
Succinate dehydrogenase3 |
0.14 ± 0.01 |
0.18 ± 0.01 |
0.15 ± 0.01 |
0.014 ± 0.002 |
0.016 ± 0.0012 |
0.014 ± 0.009 |
|||
|
Mn SOD |
19.52 ± 0.050 |
16.89 ± 0.042 |
17.28 ± 0.032 |
8.42 ± 0.012 |
6.33 ± 0.044 |
6.80 ± 0.010 |
|||
|
|