Table 4 - Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene

Table 4. Stability of bleomycin resistance in P. chrysogenum transformants.

	F1	F2	F3	F4	F5
Control	-	-	-	-	-
Transformants-p	+	+	+	+	+
Transformants-a	+	+	+	+	+

Note: +, grow; -, no grow; 20mg/ml bleomycin; Transformants-p: P. chrysogenum transformants by PEG transformation of protoplasts; Transformants-a: P. chrysogenum transformants using A. tumefaciens ; twenty strains were selected from control, transformants-p, transformants-a, respectively.